Vaccination with gamma-irradiated Neospora caninum tachyzoites protects mice against acute challenge with N. caninum |
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Authors: | Ramamoorthy S Lindsay D S Schurig G G Boyle S M Duncan R B Vemulapalli R Sriranganathan N |
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Affiliation: | Department of Biomedical Sciences and Pathobiology, Virginia Tech, 1410 Prices Fork Road, Blacksburg, Virginia 24061-0342, USA. |
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Abstract: | Neospora caninum, an apicomplexan parasite, is a leading cause of bovine abortions worldwide. The efficacy of gamma-irradiated N. caninum strain NC-1 tachyzoites as a vaccine for neosporosis was assessed in C57BL6 mice. A dose of 528 Gy of gamma irradiation was sufficient to arrest replication but not host cell penetration by tachyzoites. Female C57BL6 mice were vaccinated with two intraperitoneal inoculations of 1 x 10(6) irradiated tachyzoites at 4-wk intervals. When stimulated with N. caninum tachyzoite lysates, splenocytes of vaccinated mice, cultured 5 and 10 wk after vaccination, secreted significant (P<0.05) levels of interferon gamma, interleukin (IL)-10, and small amounts of IL-4. Antibody isotype-specific ELISA of sera from vaccinated mice exhibited both IgG1 and IgG2a isotypes of antibodies. Vaccinated mice were challenged intraperitoneally with 2 x 10(7)N. caninum tachyzoites. All vaccinated mice remained healthy and showed no obvious signs of neosporosis up to the 25th day post-challenge when the study was terminated. All unvaccinated control mice died within 1 wk of infection. Gamma-irradiated N. caninum tachyzoites can serve as an effective, attenuated vaccine for N. caninum. |
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Keywords: | Attenuation bovine abortion γ irradiation IL-10 IL-4 immune response interferon γ resistance |
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