Actin in mating structures of Chlamydomonas eugametos gametes |
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Authors: | H.W. Kalshoven J. Brederoo A.M.C. Emons A. Musgrave F. Schuring B. Wells H. van den Ende |
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Affiliation: | Dept of Molecular Cell Biology, Univ. of Amsterdom, Kruislaan 318, 1098 SM Amsterdam. The Netherlands;Dept of Plant Cytology and Morphology, Landbouwuniv. Wageningen, Arboretumlaan 4. 6703 BD Wageningen.;Dept of Cell Biology, John Innes Institute. Colney Lane, Norwich NR47UH. UK. |
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Abstract: | The presence of actin in Chlamydomonas eugametos mating structures was studied using monoclonal anti-actin antibodies. Immunofluorescent labelling of mating gametes clearly stained their mating structures and this was confirmed at the electron microscope level by immunogold labelling of sections. Anti-actin labelling also strongly stained the flagella at the flagellar collar regions and weakly stained the rest of the flagella. Treatment of gametes with 6–8% ethanol induced mating structures which protruded as large 'balloons'. Balloons stained brilliantly with anti-actin antibodies and weakly with FITC-phalloidin, a fluorescent reagent that stains F-actin. Isolated mating structure balloons and flagella were analyzed using western blotting. A prominent 43 kDa band, co-migrating with actin in erythrocyte ghosts, reacted with anti-actin antibodies. The results indicate that actin is present in mating structures and flagella of both mating types of C, eugametos . |
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Keywords: | Actin Chlamydomonas cytoskeleton mating structure |
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