DNA repair in a UV-sensitive mutant of of mouse cell line |
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Authors: | Koki Sato RB Setlow |
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Institution: | Biology Department, Brookhaven National Laboratory, Upton, NY 11973, U.S.A. |
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Abstract: | A UV-sensitive mutant, Q31, isolated from mouse-lymphoma L5178Y cells, was studied for excision and post-replication rerpairs. A nearly equal number of UV endonuclease-sensitive sites was induced by UV in L5178Y, Q31, and human Raji cells. L5178Y cells irradiated with 10 J/m2 removed 18% of sensitive sites from DNA during incubation for 24 h, and Q31 cells removed 3% of the sites, a fraction less than the limit of detection, whereas Raji cells eliminated about 60% of the sites. These results indicate that mouse-lymphoma cells are capable of excision repair to a limited extend as compared with human cells and that mutant Q31 cells are essentially devoid of dimer excision. The newly synthesized DNA was of smaller size in UV-irradiated and unirradiated Q31 cells than that in the corresponding L5178Y cells, but the DNAs in both strains increased to comparable sizes after a 2-h chase. |
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Keywords: | UV ultraviolet radiation XP xeroderma pigmentosum dThd deoxythymidine PBSS phosphate-buffered saline containing 0 5% calf serum Tris tris(hydroxymethyl)aminomethane EDTA disodium ethylenediamine-tetraacetate |
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