An optimized fed-batch culture strategy integrated with a one-step fermentation improves l-lactic acid production by Rhizopus oryzae |
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Authors: | Yongqian Fu Xiaolong Sun Huayue Zhu Ru Jiang Xi Luo Longfei Yin |
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Affiliation: | 1.Institute of Biomass Resources,Taizhou University,Jiaojiang,People’s Republic of China |
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Abstract: | In previous work, we proposed a novel modified one-step fermentation fed-batch strategy to efficiently generate l-lactic acid (l-LA) using Rhizopus oryzae. In this study, to further enhance efficiency of l-LA production through one-step fermentation in fed-batch cultures, we systematically investigated the initial peptone- and glucose-feeding approaches, including different initial peptone and glucose concentrations and maintained residual glucose levels. Based on the results of this study, culturing R. oryzae with initial peptone and glucose concentrations of 3.0 and 50.0 g/l, respectively, using a fed-batch strategy is an effective approach of producing l-LA through one-step fermentation. Changing the residual glucose had no obvious effect on the generation of l-LA. We determined the maximum LA production and productivity to be 162 g/l and 6.23 g/(l·h), respectively, during the acid production stage. Compared to our previous work, there was almost no change in l-LA production or yield; however, the productivity of l-LA increased by 14.3%. |
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