Characterization of bacterial composition and diversity in a long-term petroleum contaminated soil and isolation of high-efficiency alkane-degrading strains using an improved medium |
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| Authors: | Jun Zheng Jun-Qiao Feng Lei Zhou Serge Maurice Mbadinga Ji-Dong Gu Bo-Zhong Mu |
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| Institution: | 1.State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry,East China University of Science and Technology,Shanghai,People’s Republic of China;2.School of Biological Sciences,The University of Hong Kong,Hong Kong,People’s Republic of China;3.Shanghai Collaborative Innovation Center for Biomanufacturing Technology,Shanghai,People’s Republic of China |
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| Abstract: | Bacterial community and diversity in a long-term petroleum-contaminated soil of an oilfield were characterized using 16S rRNA gene-based Illumina MiSeq high-throughput sequencing. Results indicated that Proteobacteria (49.11%) and Actinobacteria (24.24%) were the most dominant phyla, and the most abundant genera were Pseudoxanthomonas (8.47%), Luteimonas (3.64%), Alkanindiges (9.76%), Acinetobacter (5.26%) and Agromyces (8.56%) in the soil. Meanwhile a series of cultivations were carried out for isolation of alkane degraders from petroleum-contaminated soil with gellan gum and agar as gelling agents. And the isolates were classified by their 16S rRNA genes. Nine of the isolates including Enterobacter, Pseudomonas,Acinetobacter, Rhizobium, Bacillus, Sphingomonas, Paenibacillus, Variovorax and Rhodococcus showed strong biodegradability of alkane mixture (C9–C30) in a wide range of chain-length, which could be potentially applied in enhancement of bioremediation. |
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