小伞山羊草中新型avenin-like基因的克隆及真核表达载体的构建

目的：克隆小伞山羊草中新型avenin-like（类燕麦储藏蛋白）基因,揭示avenin-like基因的表达模式,并构建avenin-like基因真核胚乳特异性表达载体。方法：利用RT-PCR方法揭示avenin-like基因的表达模式,并用PCR方法从小伞山羊草中克隆新型avenin-like基因;将克隆的avenin-like基因插入表达载体pLRPT构建真核表达载体pLRPT-avel,并经酶切和测序鉴定。结果：avenin-like基因在胚乳中特异性表达;克隆得到新型avenin-like基因,并构建了其真核胚乳特异性表达载体。结论：新型avenin-like基因的克隆及其真核表达载体的构建,为小麦品质改良提供了研究基础。

Cloning and Eukaryotic Expression Vector Construction of avenin-like Gene in Aegilops umbellulata

Objective： To clone novel avenin-like gene from Aegilops umbellulata and reveal its expression pattern, then to construct eukaryotic expression vector. Method： The expression pattern of avenin-like gene was revealed by RT-PCR, and novel avenin-like gene was amplified from A.umbelluhua by PCR, then it was cloned to vector pLRtrF. The recombi- nant plasmid was identified by sequencing and digestion with restriction enzymes. Results： avenin-like gene expressed dis- tinctively in endosperm, novel avenin-like gene was isolated and inserted to vector pLRPT. Eukaryotic expression vector pLRPT-avel was constructed. Conclusion： It should provide a foundation for quality improvement of wheat as a novel avenin-like gene was cloned and the pLRPT-avel vector was constructed.