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Enzymatic Elimination of Collagen in the Alcian Blue Staining of Connective Tissue Ground Substance
Authors:John R Ring  Dorothy Krug  Ann Dally Ogawa
Institution:  a Anatomy Department, Washington University School of Dentistry, St Louis, Mo
Abstract:Collagenase pretreatment of frozen-dried sections permits Alcian blue staining of mucopolysaccharides of connective tissue ground substance without the interference of collagen staining. Hyaluronidase elimination of Alcian blue staining confirms mucopolysaccharide as a substrate of the staining reaction.
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