Enzymatic Elimination of Collagen in the Alcian Blue Staining of Connective Tissue Ground Substance |
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Authors: | John R Ring Dorothy Krug Ann Dally Ogawa |
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Institution: |
a Anatomy Department, Washington University School of Dentistry, St Louis, Mo |
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Abstract: | Collagenase pretreatment of frozen-dried sections permits Alcian blue staining of mucopolysaccharides of connective tissue ground substance without the interference of collagen staining. Hyaluronidase elimination of Alcian blue staining confirms mucopolysaccharide as a substrate of the staining reaction. |
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