In vitro anti-HIV activity of phosphorothioate alpha-anomeric oligodeoxynucleotides |
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Authors: | B Rayner M Matsukura F Morvan J S Cohen J L Imbach |
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Affiliation: | Laboratoire de Chimie bio-organique, U.A. n 488, C.N.R.S., Université de Montpellier-II. |
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Abstract: | Oligonucleotide analogs consisting exclusively of alpha-anomeric deoxynucleoside units bridged with phosphorothioate linkages have been synthesized and tested in vitro as antiviral agents against human immunodeficiency virus (HIV) in human T cells. Two 28-mers, an homopolymer alpha-S-dC28 and an oligomer alpha-S-anti-rev complementary to the initiation site of the regulatory viral gene rev exhibited antiviral activities comparable to those reported for the corresponding beta-anomeric phosphorothioate analogs. In contrast, a nuclease-resistant homopolymer, alpha-dC28 was inactive. Their preliminary results would indicate that the origin of oligonucleotide phosphorothioate anti-HIV activity is not exclusively correlated with their higher nuclease resistance. |
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