Piercing and vacuum infiltration of the mature embryo: a simplified method for Agrobacterium-mediated transformation of indica rice |
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Authors: | Jianzhong Lin Bo Zhou Yuanzhu Yang Jin Mei Xiaoying Zhao Xinhong Guo Xingqun Huang Dongying Tang Xuanming Liu |
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Affiliation: | (1) Institute of Bioscience and Biotechnology, Hunan University, Changsha, 410082 Hunan, China;(2) Academy of Seed Industry of Hunan Yahua, Changsha, 410001 Hunan, China |
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Abstract: | Traditional transformation methods are complex and time consuming. It is generally difficult to transform indica rice varieties using traditional transformation methods due to their poor regeneration. In this contribution, a simple method was developed for the transformation of indica rice. In this method, the mature embryos of soaked seeds were pierced by a needle, and then soaked in the Agrobacterium inoculum under vacuum infiltration. The inoculated seeds germinated and grew to maturation (T 0) under nonsterile conditions. The herbicide or antibiotic analysis and molecular analysis were conducted on T 0 plants. The results showed that although the efficiency of transformation was about 6.0%, it was easier to transform indica rice using the proposed method, and the transformation process was significantly shortened. The success of transformation was further confirmed by the genetic and molecular analyses of T 1 transformants. |
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Keywords: | Agrobacterium tumefaciens Indica rice Transformation Vacuum infiltration |
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