Over-expression of ThpI from Choristoneura fumiferana enhances tolerance to cold in Arabidopsis |
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| Authors: | Bo Zhu Ai-Sheng Xiong Ri-He Peng Jing Xu Xiao-Fen Jin Xiu-Rong Meng Quan-Hong Yao |
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| Affiliation: | (1) Agro-Biotechnology Research Center, Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, 201106 Shanghai, China;(2) College of Agriculture and Biology, Shanghai Jiao Tong University, 200240 Shanghai, China;(3) College of Horticulture, Nanjing Agricultural University, 210095 Nanjing, China;(4) College of Bioscience and Biotechnology, Yangzhou University, 225009 Yangzhou, China; |
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| Abstract: | Thermal hysteresis proteins (Thps) known as antifreeze proteins for their antifreeze activity, depress the freezing point of water below the melting point in many polar marine fishes, terrestrial arthropods and plants. For the purpose of breeding cold-resistant plants, we designed to introduce the Thp gene into the plants. The physiological and biochemical effect of high-lever expression of the modified Choristoneura fumiferana Thp (ThpI) in Arabidopsis thaliana plants was analyzed. Under low temperature stress, the ThpI transgenic plants exhibited stronger growth than wild-type plants. The elevated cold tolerance of the ThpI over-expressing plants was confirmed by the changes of electrolyte leakage activity, malonyldialdehyde and proline contents. These results preliminarily showed that the Thp possibly be used to enhance the low temperature-tolerant ability of plants. |
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