| 鸡平滑肌肌球蛋白轻链激酶在NIH 3T3细胞中的表达 |
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| 引用本文： | 汪渊,桂淑玉.鸡平滑肌肌球蛋白轻链激酶在NIH 3T3细胞中的表达[J].生物化学与生物物理学报,1997,29(2):157-162. |
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| 作者姓名： | 汪渊 桂淑玉 |
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| 作者单位： | [1]安徽医科大学生化教研室 [2]安徽医科大学附属医院 |
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| 摘 要： | 肌球蛋白轻链激酶（ＭＬＣＫ）在调节平骨肌细胞收缩过程中具有十分重要的作用。本言语通过将ＭＬＣＫｃＤＮＡ插到质粒ｐＢＫｒｓｖ中构建ｐＢＫｒｓｖ－ＭＬＣＫ，并转染至ＮＩＨ３Ｔ３细胞中，ＤＮＡ－ＰＣＲ、ＲＴ－ＰＣＲ和Ｗｅｓｔｅｒｎ ｂｌｏｔ分析表达转染细胞可表达ＭＬＣＫ。活生分析表明所表达的ＭＬＣＫ具有生物学活性。为进一步研究ＭＬＣＫ在信号传导，调节平骨肌收缩等作用奠定了基础。
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| 关 键 词： | 肌球蛋白 轻链激酶 表达 NIH3T3细胞 平滑肌 |
Expression of Chicken Smooth Muscle Myosin Light Chain Kinase in NIH 3T3 Cells |
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| WANG Yuan.Expression of Chicken Smooth Muscle Myosin Light Chain Kinase in NIH 3T3 Cells[J].Acta Biochimica et Biophysica Sinica,1997,29(2):157-162. |
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| Authors: | WANG Yuan |
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| Abstract: | The myosin light chain kinase(MLCK) is important in regulating the smooth muscle contraction.The pBKrsv MLCK was obtained by inserting MLCK cDNA into pBKrsv polylinker and transfected into NIH 3T3 cells. The results revealed that the transfected cells can express chicken MLCK identified by DNA PCR, RT PCR and Western blot. The expressed MLCK has high activity, thus providing the basis for further investigation of the signal transduction and regulation of smooth muscle contraction of MLCK. |
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| Keywords: | Myosin light chain kinase(MLCK) pBKrsv Expression NIH 3T3 cells |
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