Chromosome and DNA damage analysis in individuals occupationally exposed to pesticides with relation to genetic polymorphism for CYP 1A1 gene in Ecuador |
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Authors: | Paz-y-Miño Cesar Arévalo Melissa Sanchez María Eugenia Leone Paola E |
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Institution: | Laboratorio de Genética Molecular, y Citogenética Humana, Escuela de Biología, Pontificia Universidad Católica del Ecuador, Quito, Ecuador. cpazymino@puce.edu.ec |
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Abstract: | DNA damage was measured by using the alkaline comet assay and the chromosomal aberration (CA) test using peripheral blood samples from 45 pesticide sprayers from Cayambe, Ecuador. From a total of approximately 200 nuclei scored for each donor with the comet assay, a highly significant increase in DNA migration was observed when compared with a similar unexposed control population. Additionally, in the CA test, the exposed individuals were found to be significantly different when compared to the control population. Polymorphisms for the CYP 1A1 (Msp I and Ile/Val) in exposed individuals were analyzed by PCR-RFLP and allele-specific PCR techniques. No association was found between the polymorphisms and higher levels of DNA damage as assessed by the comet assay. |
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