| 两个枣树扩展蛋白基因cDNA全序列的克隆及分析 |
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| 引用本文： | 孟玉平,曹秋芬,魏玮,孙海峰. 两个枣树扩展蛋白基因cDNA全序列的克隆及分析[J]. 生物技术通报, 2010, 0(2) |
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| 作者姓名： | 孟玉平 曹秋芬 魏玮 孙海峰 |
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| 作者单位： | 1. 山西省农业生物技术研究中心,太原,030031
2. 山西大学化学化工学院,太原,030006 |
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| 基金项目： | 山西省自然科学基金项目，山西省留学基金项目 |
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| 摘 要： | 用定向克隆法构建了枣树(Ziziphus jujubaMill.)快速生长初期结果枝的cDNA文库,经过重组质粒的筛选,克隆获得两个扩展蛋白基因cDNA全序列,分别命名为ZjEXP1(GenBank登录号:FJ449891)和ZjEXP2(GenBank登录号:FJ449892)。ZjEXP1全长1 037 bp,包含编码254个氨基酸的完整开放阅读框;ZjEXP2全长905 bp,包含编码251个氨基酸的完整开放阅读框。两个序列有共同的结构特征,即在N-末端有8个保守的半胱氨酸残基的丰富域,C-末端有4个保守的色氨酸残基的丰富域,中间有一个组氨酸(His-Phe-Asp,HFD)功能域。两者之间的氨基酸同源性为74.0%。从已知的扩展蛋白基因家族的进化分析表明,ZjEXP1和ZjEXP2由一个祖先进化而来,又分别属于两个不同的分支。
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| 关 键 词： | 枣树 扩展蛋白基因 cDNA克隆 序列分析 |
Cloning and Sequence Analysis of Two Expansin Genes cDNA from Chinese Jujube |
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| Meng Yuping,Cao Qiufen,Wei Wei,Sun Haifeng. Cloning and Sequence Analysis of Two Expansin Genes cDNA from Chinese Jujube[J]. Biotechnology Bulletin, 2010, 0(2) |
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| Authors: | Meng Yuping Cao Qiufen Wei Wei Sun Haifeng |
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| Affiliation: | Meng Yuping 1 Cao Qiufen 1 Wei Wei1 Sun Haifeng2(1 Agri-Biotechnology Research Center of Shanxi Province,Taiyuan 030031,2College of Chemistry , Chemical Engineering of Shanxi University,Taiyuan 030006) |
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| Abstract: | Using directional cloning,a cDNA library of the bearing shoot during early days of rapid growth in Chinese jujube was constructed.Two expansin genes were obtained through plasmid recombination and clone technique,which were named as ZjEXP1(GenBank Number:FJ449891)and ZjEXP2(GenBank Number:FJ449892),respectively.Sequence analysis indicated that ZjEXP1 is 1 037 bp in full length and contains an encoding 254 amino acids open reading frame,and that ZjEXP2 is 905 bp in full length and contains an encoding 251 am... |
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| Keywords: | Ziziphus jujuba Mill. Expansin gene cDNA cloning Sequence analysis |
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