真菌中G蛋白信号调控因子蛋白类型与其理化性质的关系

目的]G蛋白信号调控因子(RGS)作为G蛋白信号转导途径的负调控因子,在植物病原菌的致病性和有性生殖调控方面发挥着重要作用.研究真菌中RGS蛋白类型与其理化性质及特征的关系,为今后深入开展不同真菌中具有不同类别RGS的功能解析打下坚实的理论基础.方法]前期对模式生物、病原菌、非致病菌等49个真菌中229个RGS蛋白...

Relationship between RGS protein types and physicochemical properties in fungi

Objective] As a negative regulator of G protein signal transduction pathway, G protein signal regulator factor plays an important role in the pathogenicity asexual reproduction regulation of plant pathogens. To identify the relationship between RGS protein types and their physicochemical properties in fungi. Methods] We analyzed 229 of previously identified RGS protein sequences in 49 fungi including model organism, pathogenic bacteria and non-pathogenic bacteria, and identified 6 proteins according to their conserved domains, such as DEP-RGS, RGS-TM, PXA-RGS-PX, RGS, RGS-PAS-PAC and TM-RGS. We used the protein database, ProtComp v9.0, PHD and MEME to analyze the physicochemical properties, subcellular localization, secondary structure and motif of the above RGS proteins. Results] The above-mentioned different types of RGS proteins had obvious characteristic futures, and following common characteristics: theoretical isoelectric point was between 6.01 and 7.00, instability coefficients were between 40.01 and 60.00, more than 95% of RGS proteins were hydrophilic protein, the strongest hydrophilic amino acid residues were arginine, aspartic acid, glutamic acid, glutamine, asparagine, the strongest hydrophobic amino acid residues were leucine, alanine, valine, phenylalanine, isoleucine, the secondary structure was characterized by less Beta strand, situation for the transit peptide situation is unclear, and subcellular location is more concentrated in the nucleus. Conclusion] The physicochemical properties of the six RGS proteins have some common characteristics, but different types of RGS proteins also have obvious category characteristics, mainly in the conserved domain, secondary structure, isoelectric point, transport peptide and subcellular localization.