印度梨形孢对铁皮石斛种子萌发和原球茎生长的影响

为探究印度梨形孢(Piriformospora indica)对铁皮石斛(Dendrobium officinale)种子萌发和原球茎生长的影响,在铁皮石斛种子离体培养和原球茎生长阶段分别接种印度梨形孢,对其形态发育特征和生理特性进行研究.结果表明,接种印度梨形孢的铁皮石斛种子的起始萌发时间提前,接种印度梨形孢的铁皮石...

Effects of Piriformospora indica on Seed Germination and Protocorm Growth of Dendrobium officinale

In order to explore the effects of Piriformospora indica on seed germination and protocorm growth of Dendrobium officinale, the morphological and physiological characteristics were studied inoculated by P. indica on seeds and protocorm. The results showed that the initial germination time of D. officinale seeds inoculated with P. indica was earlier than the control, and the height, fresh weight, dry weight, number of leaves, leaf length, leaf width, root number and root length of protocorm of D. officinale inoculated with P. indica were higher than the control. The seed germination rate was 68.8% after 60 days of inoculation, which was higher than the control (28.6%). The chlorophyll a content, chlorophyll b content and total chlorophyll content in the protocorm of D. officinale inoculated for 80 days were significantly higher than the control, chlorophyll a/b was lower than the control, the net photosynthesis rate, stomatal conductance and transpirationrate were higher than the control, and the content of medicinal polysaccharide was significantly higher than the control. Therefore, the seed germination and protocorm growth of D. officinale were promoted by inoculation, and the quality and yield were improved.