| 蔗叶堆肥中一株泡盛曲霉溶磷能力的鉴定及其对辣椒的促生效果 |
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| 引用本文： | 赖鉴添,杨婷,史发超,和锐敏,王鑫,邵丹青,何琴,卢颖林,胡黎明,安玉兴. 蔗叶堆肥中一株泡盛曲霉溶磷能力的鉴定及其对辣椒的促生效果[J]. 微生物学报, 2021, 61(1): 77-91 |
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| 作者姓名： | 赖鉴添 杨婷 史发超 和锐敏 王鑫 邵丹青 何琴 卢颖林 胡黎明 安玉兴 |
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| 作者单位： | 仲恺农业工程学院资源与环境学院, 广东 广州 510225;广东省科学院生物工程研究所, 广东省药肥工程技术研究中心, 广东 广州 510316;广东省科学院生物工程研究所, 广东省药肥工程技术研究中心, 广东 广州 510316;华南农业大学农学院, 广东 广州 510642 |
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| 基金项目： | 广东省重点领域研发计划（2018B020205003）；国家重点研发计划（2018YFD0201100）；广东省科技计划（2016A020221018）；现代农业产业技术体系建设专项资金（CARS-170306） |
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| 摘 要： | [目的]从甘蔗叶堆肥中分离筛选具有高效溶磷及促生功能的菌株,为微生物肥料制备提供一种可利用的菌种资源.[方法]以Ca3(PO4)2和Zn3(PO4)2为磷源,进行平板溶磷筛选实验;采用形态学特征和ITS rDNA序列分析法进行菌种鉴定;采用液体摇瓶培养测定菌株的溶磷能力;将溶磷菌接种至辣椒幼苗根部分析其促生效应.[结果...
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| 关 键 词： | 溶磷真菌 泡盛曲霉 溶磷作用 促生作用 |
| 收稿时间： | 2019-12-31 |
| 修稿时间： | 2020-03-11 |
A phosphorus-dissolving Aspergillus awamori strain from sugarcane leaf compost for growth promotion of pepper |
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| Jiantian Lai,Ting Yang,Fachao Shi,Ruimin He,Xin Wang,Danqing Shao,Qin He,Yinglin Lu,Liming Hu,Yuxing An. A phosphorus-dissolving Aspergillus awamori strain from sugarcane leaf compost for growth promotion of pepper[J]. Acta microbiologica Sinica, 2021, 61(1): 77-91 |
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| Authors: | Jiantian Lai Ting Yang Fachao Shi Ruimin He Xin Wang Danqing Shao Qin He Yinglin Lu Liming Hu Yuxing An |
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| Affiliation: | College of Resources and Environment, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, Guangdong Province, China;Guangdong Province Pharmaceutical Fertilizer Engineering Technology Research Center, Institute of Bioengineering, Guangdong Academy of Sciences, Guangzhou 510316, Guangdong Province, China;Guangdong Province Pharmaceutical Fertilizer Engineering Technology Research Center, Institute of Bioengineering, Guangdong Academy of Sciences, Guangzhou 510316, Guangdong Province, China;College of Agriculture, South China Agricultural University, Guangzhou 510642, Guangdong Province, China |
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| Abstract: | [Objective] Isolation of strains with high-efficiency of phosphorus-dissolving and growth-promoting functions from sugarcane leaf compost has provided available microbial resources for bio-fertilizers production. [Methods] Phosphorus-dissolving strains were screened from insoluble inorganic phosphorus media containing Ca3(PO4)2 or Zn3(PO4)2, and identified by morphological characteristics and ITS rDNA sequence analysis. The phosphorus-dissolving ability of the strains was determined by the halo zone and molybdenum antimony colorimetric methods, and the growth-promoting ability of the strain was analyzed by pot experiment of pepper. [Results] Strain DC30-2-P1 was screened from the compost and identified as Aspergillus awamori. The diameter of the colony of the strains (d) was 55.33 mm and 45.00 mm, the halo zone (D) was 65.33 mm and 67.67 mm, and the D/d value was 1.16 and 1.50 on the medium with Ca3(PO4)2 and Zn3(PO4)2, respectively. After 96 hours of cultivation in a liquid medium with Ca3(PO4)2 as the phosphorus source, the available phosphorus content reached 2974 mg/L. The results indicated that strain DC30-2-P1 had a significant effect on dissolving phosphorus. The results of the pot experiment show that the inoculation of DC30-2-P1 promoted the growth of pepper. Compared with the treatment group without phosphate solubilizing fungi, the above-ground fresh and dry weight increased by 18.6% and 43.5%, the under-ground fresh and dry weight increased by 30.2% and 25%, plant height increased by 13.6%, and chlorophyll content increased by 44.9%, both plant total phosphorus content and soil available phosphorus content increased by more than 80%. [Conclusion] Strain DC30-2-P1 from sugarcane leaf compost has a high efficiency on dissolving insoluble phosphorus compounds, promoting the absorption of phosphorus and improving crop yield. |
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| Keywords: | phosphate-solubilizing fungus Aspergillus awamori phosphate-dissolving effect growth-promoting effect |
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