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Applications of direct detection device in transmission electron microscopy
Authors:Jin Liang  Milazzo Anna-Clare  Kleinfelder Stuart  Li Shengdong  Leblanc Philippe  Duttweiler Fred  Bouwer James C  Peltier Steven T  Ellisman Mark H  Xuong Nguyen-Huu
Institution:aUniversity of California at San Diego, La Jolla, CA 92093, USA;bUniversity of California at Irvine, Irvine, CA 92697, USA
Abstract:A prototype direct detection device (DDD) camera system has shown great promise in improving both the spatial resolution and the signal to noise ratio for electron microscopy at 120–400 keV beam energies (Xuong et al., 2007. Methods in Cell Biology, 79, 721–739). Without the need for a resolution-limiting scintillation screen as in the charge coupled device (CCD), the DDD camera can outperform CCD based systems in terms of spatial resolution, due to its small pixel size (5 μm). In this paper, the modulation transfer function (MTF) of the DDD prototype is measured and compared with the specifications of commercial scientific CCD camera systems. Combining the fast speed of the DDD with image mosaic techniques, fast wide-area imaging is now possible. In this paper, the first large area mosaic image and the first tomography dataset from the DDD camera are presented, along with an image processing algorithm to correct the specimen drift utilizing the fast readout of the DDD system.
Keywords:Direct detection device  Active pixel sensor  Image processing algorithm  Electron tomography  Cryo-electron microscopy  Cryo-electron tomography
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