Efficient release of overproduced gene products from Escherichia coli BL21(DE3) by lytic infection with newly isolated bacteriophages |
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| Authors: | Iida Yuichiro Matsuda Yoshinori Saito Ryuichiro Nakasato Masanori Nonomura Teruo Kakutani Koji Tosa Yukio Mayama Shigeyuki Toyoda Hideyoshi |
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| Affiliation: | Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University, Nara 631-8505, Japan. |
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| Abstract: | Overproduced proteins from Escherichia coli BL21(DE3) were efficiently released with virulent bacteriophages. Leviviridae-like bacteriophages were isolated from soil and used to lyse BL21(DE3) cells transformed with beta-glucosidase, chitinase, or chitosanase genes. This method caused lysis of bacterial cells similar to that by conventional sonication and enabled us to effectively recover and purify the enzymes. |
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