Isolation and characterization of a spontaneously immortalized bovine retinal pigmented epithelial cell line |
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| Authors: | Thomas E Liggett T Daniel Griffiths and Elizabeth R Gaillard |
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| Institution: | (1) Department of Biological Sciences, Northern Illinois University, DeKalb, IL, USA;(2) Department of Neurological Sciences, Rush University Medical Center, Chicago, IL, USA;(3) Department of Biological Sciences, Marquette University, Milwaukee, WI, USA;(4) Department of Chemistry and Biochemistry, Northern Illinois University, DeKalb, IL, USA |
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| Abstract: | Background The Retinal Pigmented Epithelium (RPE) is juxtaposed with the photoreceptor outer segments of the eye. The proximity of the
photoreceptor cells is a prerequisite for their survival, as they depend on the RPE to remove the outer segments and are also
influenced by RPE cell paracrine factors. RPE cell death can cause a progressive loss of photoreceptor function, which can
diminish vision and, over time, blindness ensues. Degeneration of the retina has been shown to induce a variety of retinopathies,
such as Stargardt's disease, Cone-Rod Dystrophy (CRD), Retinitis Pigmentosa (RP), Fundus Flavimaculatus (FFM), Best's disease
and Age-related Macular Degeneration (AMD). We have cultured primary bovine RPE cells to gain a further understanding of the
mechanisms of RPE cell death. One of the cultures, named tRPE, surpassed senescence and was further characterized to determine
its viability as a model for retinal diseases. |
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