Simple and rapid determination of the activity of recombinant D-amino acid oxidase in cephalosporin C bioconversion with use of a micro pO2 probe

Summary D-Amino acid oxidase (DAAO) genes were amplified from Trigonopsis variabilis and Rhodotorula gracilis by polymerase chain reactions. T. variabilis DAAO gene was cloned into a pUC19 vector. The expression of the T. variabilis DAAO was directly determined in permeabilized E. coli cells using a micro pO₂ probe. The micro pO₂ probe was sensitive enough to be suitable for a simple and rapid estimation of DAAO activity toward cephalosporin C.