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The nature of sialic acids in human lymphocytes
Authors:Johannis P Kamerling  Josef Makovitzky  Roland Schauer  Johannes FG Vliegenthart  Margret Wember
Institution:1. Department of Bio-Organic Chemistry, State University of Utrecht, Croesestraat 79, 3522 AD Utrecht, The Netherlands;2. National Institute for Rheumatism and Physiotherapy, Department of Pathology, 1525 Budapest, Hungary;3. Biochemisches Institut der Christian-Albrechts-Universität, Olshausenstrasse 40-60, 2300 Kiel 1, F.R.G.
Abstract:Analysis of the sialic acids obtained by mild acid hydrolysis of B lymphocytes reveals the presence of N-acetylneuraminic acid and 9-O-acetyl-N-acetylneuraminic acid. For T lymphocytes only N-acetylneuraminic acid has been demonstrated to occur. The applied methods include quantitative colorimetry, thin-layer chromatography and combined gas-liquid chromatography-mass spectrometry.
Keywords:O-Acetylation  Neuraminic acid  Sialic acid  Carbohydrate analysis  (Human lymphocyte)
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