Localization of neutral,magnesium-stimulated sphingomyelinase in plasma membrane of cultured neuroblastoma cells |
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| Authors: | Matthew W Spence Jan Wakkary Joe TR Clarke Harold W Cook |
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| Institution: | Departments of Pediatrics and Biochemistry and Atlantic Research Centre for Mental Retardation, Dalhousie University, Halifax, Nova Scotia, B3H 4H7 Canada |
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| Abstract: | A parallel is shown between the distribution of neutral sphingomyelinase and plasma membrane enzymes (5′-nucleotidase and (Na+ + K+)-activated ATPase) in cultured neuroblastoma cells. In contrast there is no evidence of localization in lysosomes (β-hexosaminidase and acid sphingomyelinase), mitochondria (carnitine palmitoyltransferase), or cytosol. Activity in the microsomal fraction is attributed primarily to plasma membrane contamination. |
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| Keywords: | Sphingomyelinase Subcellular localization Plasma membrane (Neuroblastoma cell) |
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