A method for fractionation of cloned protein in recombinantSaccharomyces cerevisiae |
| |
Authors: | Mark Marten Jin-Ho Seo |
| |
Affiliation: | (1) School of Chemical Engineering, Purdue University, 47907 West Lafayette, Indiana |
| |
Abstract: | In a spheroplasting method which allows the fractionation and quantification of cloned invertase activity in recombinantSaccharomyces cerevisiae cells, the yeast cell is selectively degraded with the enzyme Zymolyase for 60 minutes at 45°C to separate periplasmic proteins from cytoplasmic proteins. Most of the glucose-6-phosphate dehydrogenase (a cytoplasmic marker protein) was found in the cytoplasmic fraction. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|