| 运用BioID技术筛选S100A7互作蛋白* |
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| 引用本文： | 王俊豪,孔菲,李允广,刘进,胡恩泽,王睿,黄凌云,肖雪媛. 运用BioID技术筛选S100A7互作蛋白*[J]. 中国生物工程杂志, 2022, 42(8): 52-62. DOI: 10.13523/j.cb.2203048 |
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| 作者姓名： | 王俊豪 孔菲 李允广 刘进 胡恩泽 王睿 黄凌云 肖雪媛 |
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| 作者单位： | 北京师范大学生命科学学院 细胞增殖及调控生物学教育部重点实验室 北京 100875 |
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| 基金项目： | * 国家重点基础研究计划(2011CB915504);国家自然科学基金(81172216) |
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| 摘 要： | 目的: 通过邻近生物素鉴定(BioID)技术筛选S100A7互作蛋白并进行验证。方法: 采用邻近生物素BioID和质谱相结合的方法,筛选S100A7互作蛋白。通过免疫荧光和免疫共沉淀方法,对相互作蛋白进行验证。结果: 与对照组比对,通过BioID技术共获得94个可能与 S100A7 相互作用的候选蛋白质。选取 Annexin A2(AnxA2)蛋白在 HEK293 细胞中进行了验证,结果表明S100A7 与 AnxA2 存在直接的相互作用,且二者共定位于细胞质和细胞膜。结论: 可将BioID技术作为互作蛋白筛选的一项新技术,通过该技术发现S100A7和AnxA2存在相互作用。
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| 关 键 词： | S100A7 BioID Annexin A2 互作蛋白 |
| 收稿时间： | 2022-03-21 |
Screening of S100A7 Interacting Proteins Using BioID Technology |
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| WANG Jun-hao,KONG Fei,LI Yun-guang,LIU Jin,HU En-ze,WANG Rui,HUANG Ling-yun,XIAO Xue-yuan. Screening of S100A7 Interacting Proteins Using BioID Technology[J]. China Biotechnology, 2022, 42(8): 52-62. DOI: 10.13523/j.cb.2203048 |
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| Authors: | WANG Jun-hao KONG Fei LI Yun-guang LIU Jin HU En-ze WANG Rui HUANG Ling-yun XIAO Xue-yuan |
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| Abstract: | Objective: To investigate and verify the candidate interactors of S100A7. Methods: The candidate interactors of S100A7 are identified using BioID and mass spectrum and verified by Co-IP and immunofluorescence. Results: 94 candidate proteins are identified in two groups by mass spectrum. Interaction between Annexin A2(AnxA2) and S100A7 was then verified using Co-IP in HEK293WT cells. It is demonstrated that AnxA2 interacts with S100A7 directly, and the immunofluorescence results show that S100A7 and AnxA2 are collocating in the cytoplasm. Conclusion: BioID is a new technology for screening S100A7 interactors and the interaction between S100A7 and AnxA2 is discovered using BioID. |
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| Keywords: | S100A7 BioID Annexin A2 Interaction proteins |
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