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Molecular cloning,expression and antioxidant characterisation of a typical thioredoxin gene (AccTrx2) in Apis cerana cerana |
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| Authors: | Pengbo Yao Lili Hao Fang Wang Xiaobo Chen Yan Yan Xingqi Guo Baohua Xu |
| Affiliation: | 1. State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China;2. College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong 271018, PR China |
| Abstract: | |
| Keywords: | ROS, reactive oxygen species Trxs, thioredoxins UTR, untranslated region ORF, open reading frame RACE-PCR, rapid amplification of cDNA ends PCR qRT-PCR, quantitative real-time PCR IPTG, isopropyl-1-thio-β-galactopyranoside EDTA, ethylenediaminetetraacetic acid SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis HEPES, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid MFO, mixed-function oxidation DTT, dithiothreitol NTA, nitrilotriacetic acid |
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