The association of MDR1 C3435T and G2677T/A polymorphisms with plasma platelet-activating factor levels and coronary artery disease risk in Turkish population |
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Authors: | Gulsel Ayaz Bahadir Batar Gonul Kanigur Mehmet Guven Ilhan Onaran Bilgehan Karadag Baris Ilerigelen Ahmet Dirican Turgut Ulutin |
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Affiliation: | 1. Department of Medical Biology, Cerrahpasa Medical Faculty, University of Istanbul, Istanbul, Turkey;2. Department of Cardiology, Cerrahpasa Medical Faculty, University of Istanbul, Istanbul, Turkey;3. Department of Biostatistics, Istanbul Medical Faculty, University of Istanbul, Istanbul, Turkey |
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Abstract: | Increased levels of peripheral proinflammatory mediators can contribute to the development of coronary artery disease (CAD). Platelet activating factor (PAF) is an important proinflammatory mediator and plasma levels of PAF correlate with transmembrane transporter multidrug resistant 1 P-glycoprotein (MDR1 Pgp) expression and activity. MDR1 polymorphisms can affect the expression and activity of Pgp and plasma PAF levels. Therefore, we investigated the possible relationship between MDR1 C3435T and G2677T/A polymorphisms and plasma PAF levels and the risk of CAD. The study population consisted of 198 patients angiographically documented CAD, including 113 cases with at least 1 coronary artery with ≥ 50% luminal diameter stenosis and 85 control subjects with strictly normal coronary angiograms. Genotypes of the MDR1 C3435T and G2677T/A polymorphisms were determined by polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP). Plasma PAF levels were detected by enzyme-linked immunosorbent assay (ELISA). There were no significant differences among plasma PAF levels in regard to MDR1 C3435T and G2677T polymorphisms in CAD patients and controls. No statistically significant difference was found for the genotypic and allelic distributions of the polymorphisms in the MDR1 gene between the patients and the control subjects. Furthermore, analysis of MDR1 haplotypes did not show any associations with increased plasma PAF levels and risk of CAD. Our results suggest that plasma PAF levels are not associated with MDR1 gene polymorphisms. There is no association between MDR1 C3435T and G2677T/A polymorphisms and the risk of CAD in Turkish patients. |
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Keywords: | CAD, coronary artery disease PAF, platelet activating factor MDR1, multidrug-resistance 1 P-gp, P-glycoprotein PCR, polymerase chain reaction RFLP, restriction fragment length polymorphism ELISA, enzyme-linked immunosorbent assay |
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