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Molecular cloning and expression pattern of 11 genes involved in lipid metabolism in yellow catfish Pelteobagrus fulvidraco
Authors:Jia-Lang Zheng  Zhi LuoQing-Ling Zhu  Xiao-Ying TanQi-Liang Chen  Lin-Dan SunWei Hu
Institution:Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., Fishery College, Huazhong Agricultural University, Wuhan 430070, China; Freshwater Aquaculture Collaborative Innovative Centre of Hubei Province, Wuhan 430070, China
Abstract:11 genes involved in lipid metabolism were cloned from liver of yellow catfish Pelteobagrus fulvidraco, including CPT 1A, CPT 1B, PPARα, PPARγ, SREBP-1, G6PD, 6PGD, FAS, acetyl-CoA ACCa, ACCb, and LPL. Phylogenetic analysis further identified these genes, and confirmed the classification and evolutionary status of yellow catfish. mRNA of all eleven genes was present in liver, muscle, mesenteric adipose, ovary and heart, but at varying levels. The present study will facilitate further studies on the regulation of lipid metabolism at the molecular level for the fish species.
Keywords:ACC  acetyl-CoA carboxylase  CPT I  carnitine palmitoyltransferase  EF1α  elongation factor 1 alpha  FAS  fatty acid synthase  GAPDH  glyceraldehydes 3-phosphate dehydrogenase  G6PD  glucose 6-phosphate dehydrogenase  HPRT  hypoxanthine-guanine phosphoribosyl transferase  LPL  lipoprotein lipase  MS-222  tricaine methanesulfonate  NJ  neighbor-joining  ORF  open reading frame  PCR  polymerase chain reaction  6PGD  6-phosphogluconate dehydrogenase  PPAR  peroxisome proliferators-activated receptor  SEM  standard error of mean  SREBP  sterol-regulator element-binding protein
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