Association of adiponectin (AdipoQ) and sulphonylurea receptor (ABCC8) gene polymorphisms with Type 2 Diabetes in North Indian population of Punjab |
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Authors: | Kawaljit Matharoo Punit Arora A.J.S Bhanwer |
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Affiliation: | 1. Department of Human Genetics, Guru Nanak Dev University, Amritsar, Punjab 143005, India;2. Diabetic Clinic and Research Institute, 171, Green Avenue, Amritsar, Punjab 143002, India |
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Abstract: | In Type 2 Diabetes (T2D), adiponectin (AdipoQ) and sulphonylurea receptor genes (ABCC8) are important targets for candidate gene association studies. The single nucleotide polymorphisms (SNPs) in these genes have been associated with features of the metabolic syndrome across various populations. The present case–control study undertaken in the population of Punjab, evaluates the association of + 45T>G polymorphism in AdipoQ gene; and Exon16-3C>T as well as Exon18C>T polymorphisms in ABCC8 gene with T2D. These SNPs were genotyped in 200 T2D cases and 200 non-diabetic healthy controls using the PCR-RFLP method. The frequency of the minor G-allele for AdipoQ+ 45(T>G) polymorphism was significantly higher in T2D cases (29.0%) than in controls (21.5%) [P = 0.02, OR = 1.49 (1.07–2.04)]. The genetic model analysis revealed that the G-allele cumulatively provides nearly 1.59–1.78 fold increased risk to T2D under the additive (P = 0.009; OR = 1.59, 1.12–2.25 at 95% CI), dominant (TG/GG vs. TT) (P = 0.034, OR = 1.64, 1.04–2.56 at 95% CI) and codominant model (TG vs. TT/GG) (P = 0.014; OR = 1.78, 1.12–2.82 at 95% CI) after adjusting for confounding factors. However, no difference in the distribution of genotype and allele frequencies was observed for both the ABCC8 polymorphisms. The distribution of obesity profiles (BMI, WC and WHR) was also significantly different between cases and controls (P < 0.05). Higher BMI and central obesity were observed to increase the risk of T2D. G-allele of + 45(T>G) polymorphism in the adiponectin gene appears to be associated with increased risk of T2D, but the polymorphisms in sulphonylurea receptor gene do not seem to be associated with T2D in the population of Punjab. |
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Keywords: | ABCC8/SUR1, Sulphonylurea receptor AdipoQ, Adiponectin ADA, American Diabetic Association ANOVA, Analysis of Variance BMI, Body Mass Index CI, Confidence Interval CAD, Coronary Artery Disease DBP, Diastolic Blood Pressure EDTA, Ethylene diamine tetra acetic acid HWE, Hardy-Weinberg Equilibrium IR, Insulin Resistance IDF, International Diabetes Federation LD, Linkage Disequilibrium OR, Odds Ratio PCR-RFLP, Polymerase Chain Reaction-Restriction Fragment Length Polymorphism SNP, Single Nucleotide Polymorphism SD, Standard Deviation SPSS, Statistical Package for the Social Sciences SBP, Systolic Blood Pressure TZD, Thiazolidinediones T2D, Type 2 Diabetes WC, Waist Circumference WHR, Waist Hip Ratio |
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