Characterization of Cdc2 kinase in the red claw crayfish (Cherax quadricarinatus): Evidence for its role in regulating oogenesis |
| |
| Authors: | Lan-mei Wang Di Zuo Wei-wei Lv Dan-li Wang A-jing Liu Yunlong Zhao |
| |
| Affiliation: | 1. Life Science College, East China Normal University, Shanghai 200062, China;2. College of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China |
| |
| Abstract: | Cdc2 kinase is a catalytic subunit of the maturation-promoting factor (MPF), a central factor for inducing the meiotic maturation of oocytes. MPF has been studied in a wide variety of animal species; however, its expression in crustaceans is poorly characterized. In this study, a complete cDNA sequence of Cdc2 kinase was cloned from the red claw crayfish, Cherax quadricarinatus, and its spatiotemporal expression profiles were analyzed. The Cdc2 cDNA (1769 bp) encodes for a 299 amino acid protein with a calculated molecular weight of 34.7 kDa. Quantitative real-time PCR demonstrated that Cdc2 mRNA was expressed mainly in the ovary tissue and the expression decreased as the ovaries developed. Immunohistochemistry analysis revealed that the Cdc2 protein relocated from the cytoplasm to the nucleus during oogenesis. These findings suggest that Cdc2 kinase may play an important role in the gametogenesis and gonad development in C. quadricarinatus. |
| |
| Keywords: | Cdc2, cyclin-dependent kinase 2 MPF, maturation-promoting factor GVBD, germinal vesicle breakdown GSI, gonadosomatic index PBS, phosphate buffered saline IHC, immunohistochemical RT-PCR, real-time reverse transcription polymerase chain reaction qRT-PCR, quantitative real-time reverse transcription polymerase chain reaction RACE, rapid amplification of cDNA ends S.E., standard error ORF, open reading frame UTR, untranslated region DP, degenerate primers |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
