Potential use of DNA barcoding for the identification of Salvia based on cpDNA and nrDNA sequences |
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| Authors: | Meng Wang Hong-xia Zhao Long Wang Tao Wang Rui-wu Yang Xiao-li Wang Yong-hong Zhou Chun-bang Ding Li Zhang |
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| Institution: | 1. College of Life and Basic Science, Sichuan Agricultural University, Yaan 625014, China;2. College of Life Science and Biotechnology, Mianyang Normal University, Mianyang 621000, China;3. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China;4. Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Wenjiang 611130, China |
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| Abstract: | An effective DNA marker for authenticating the genus Salvia was screened using seven DNA regions (rbcL, matK, trnL–F, and psbA–trnH from the chloroplast genome, and ITS, ITS1, and ITS2 from the nuclear genome) and three combinations (rbcL + matK, psbA–trnH + ITS1, and trnL–F + ITS1). The present study collected 232 sequences from 27 Salvia species through DNA sequencing and 77 sequences within the same taxa from the GenBank. The discriminatory capabilities of these regions were evaluated in terms of PCR amplification success, intraspecific and interspecific divergence, DNA barcoding gaps, and identification efficiency via a tree-based method. ITS1 was superior to the other marker for discriminating between species, with an accuracy of 81.48%. The three combinations did not increase species discrimination. Finally, we found that ITS1 is a powerful barcode for identifying Salvia species, especially Salvia miltiorrhiza. |
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| Keywords: | cpDNA chloroplast DNA nrDNA nuclear ribosomal DNA ITS internal transcribed spacer PCR polymerase chain reaction K2P Kimura 2-Parameter NJ neighbor-joining BS bootstrap support CBOL Consortium for the Barcode of Life |
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