Association of prostate cancer susceptibility variant (MSMB) rs10993994 with risk of spermatogenic failure |
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Authors: | Wei Wu Jing Lu Beilei Yuan Yufeng Qin Minjian Chen Xiaobing Niu Bin Xu Chuncheng Lu Yankai Xia Daozhen Chen Jiahao Sha Xinru Wang |
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Affiliation: | 1. State Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing 210029, China;2. Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China;3. Wuxi Maternal and Child Health Hospital Affiliated to Nanjing Medical University, Wuxi 214002, China;4. Department of Urology, Huai''an First Affiliated Hospital of Nanjing Medical University, Huai''an, China;5. Department of Urology, Affiliated Zhongda Hospital, Southeast University, Nanjing, China |
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Abstract: | β-Microseminoprotein (MSMB) is one of the most abundant proteins in human seminal plasma. It has been identified that MSMB increased significantly in oligoasthenoteratozoospermic patients compared with fertile controls. We hypothesized that the functional polymorphism (rs10993994) of MSMB gene could be a risk factor for spermatogenic failure. For this study, 338 patients with idiopathic oligozoospermia or azoospermia and 382 fertile controls were recruited from an infertility clinic. Semen analysis was performed by computer-assisted semen analysis system. The functional polymorphism of MSMB gene was genotyped using TaqMan method. Sixty three seminal plasma samples were used to test the expression of MSMB by enzyme-linked immunosorbent assay (ELISA). The TT genotype and T allele were associated with an increased risk of idiopathic infertility with azoospermia (TT genotype: OR, 1.75; 95% CI, 1.03–2.95; T allele: OR, 1.34; 95% CI, 1.03–1.75). However, no differences were found in risk for the TT genotype or T allele among men with oligozoospermia. In addition, idiopathic infertile males have significantly higher MSMB expression levels than fertile controls. We present the first epidemiologic evidence supporting the involvement of common genetic polymorphism in MSMB gene in spermatogenic failure. These results suggest that men carrying the variant have an increased risk of spermatogenic failure associated with male infertility. Further studies are needed to confirm the roles of the polymorphism in idiopathic azoospermia and investigate the biological mechanism of elevated MSMB expression in infertile males. |
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Keywords: | OR, odds ratios 95% CI, 95% confidence intervals |
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