Molecular characterization of major and minor rDNA repeats and genetic variability assessment in different species of mahseer found in North India |
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Authors: | Ravindra Kumar Mamta Singh Basdeo Kushwaha N.S. Nagpure Indra Mani W.S. Lakra |
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Affiliation: | National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow 226 002, U.P., India |
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Abstract: | Relationship among the mahseer species (Family: Cyprinidae) has long been debated in fish systematics. Present study concentrates on the nature of the phylogenetic relationship among the five mahseer species using the sequence of major ribosomal DNA (45S rDNA). We have covered rDNA sequence of approximately 5.2 kb per individual, 26.0 kb per species and 130.0 kb as a whole. We also characterized the 45S and 5S rDNA regions with respect to their nucleotide composition. For phylogenetic analyses, nucleotide sequences were divided into four datasets. First and second datasets contained 18S rDNA and ITS1 sequence, whereas third and fourth datasets consisted of ITS2 and complete 18S-ITS1-5.8S-ITS2-28S, respectively. The NJ tree was constructed for all the datasets. The mahseer species under study formed a monophyletic group well separated from the outgroup species. Similarly, the individuals of Neolissochilus hexagonolepis form monophyletic group with Tor species, indicating Neolissochilus as a sister genus of Tor. The findings from the present study provide greater insights into taxonomic status of mahseer, and set the stage for future investigations dealing with phylo-geography, taxonomy, conservation and co-evolution within this interesting and important group of fish. |
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Keywords: | AgNO3, silver nitrate AIC, Akaike Information Criterion BCIP, 5-bromo-4-chloro-3&prime -indolyphosphate p-toluidine salt CMA3, Chromomycin A3 DNA, deoxyribonucleic acid dNTPs, deoxynucleoside triphosphates EMBL, European Molecular Biology Laboratory FISH, fluorescence in situ hybridization IE, intermediate element ITS1, internal transcribed spacer 1 ITS2, internal transcribed spacer 2 KCl, Potassium chloride MgCl2, Magnesium chloride NBT, nitro-blue tetrazolium chloride NCBI, National Center for Biotechnology Information NJ, neighbor-joining NH, Neolissochilus hexagonolepis NTS, Non-transcribed spacer PCR, polymerase chain reaction rDNA, ribosomal deoxyribonucleic acid SM, supplementary materials TC, Tor chelynoides TP, Tor putitora TPr, Tor progeneius TT, Tor tor Tris&ndash HCl, Tris hydrochloride |
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