Characterization of the complete mitochondrial genome of Diaphania pyloalis (Lepidoptera: Pyralididae) |
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Authors: | Bao-Jian Zhu Qiu-Ning Liu Li-Shang Dai Lei WangYu Sun Kun-Zhang LinGuo-Qing Wei Chao-Liang Liu |
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Institution: | College of Life Science, Anhui Agricultural University, 130 Changjiang West Road, 230036, PR China |
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Abstract: | The complete mitochondrial genome (mitogenome) of Diaphania pyloalis (Lepidoptera: Pyralididae) was determined to be 15,298 bp and has the typical gene organization of mitogenomes from lepidopteran insects. It consists of 13 protein-coding genes (PCGs), two rRNA genes, 22 tRNA genes and an A + T-rich region. The A + T content of this mitogenome is 80.83% and the AT skew is slightly positive. All PCGs are initiated by ATN codons, except for cytochrome c oxidase subunit 1 (cox1) gene which is initiated by CGA. Only the cox2 gene has an incomplete stop codon consisting of just a T. All the tRNA genes display a typical clover-leaf structure of mitochondrial tRNA. The A + T-rich region of the mitogenome is 332 bp in length, including several common features found in lepidopteran mitogenomes. Phylogenetic analysis showed that the D. pyloalis is close to Pyralididae. |
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Keywords: | PCR polymerase chain reaction ATPase adenosine triphosphatase NADH nicotinamide adenine dinucleotide hydrogen |
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