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Characterization of the complete mitochondrial genome of Diaphania pyloalis (Lepidoptera: Pyralididae)
Authors:Bao-Jian Zhu  Qiu-Ning Liu  Li-Shang Dai  Lei WangYu Sun  Kun-Zhang LinGuo-Qing Wei  Chao-Liang Liu
Institution:College of Life Science, Anhui Agricultural University, 130 Changjiang West Road, 230036, PR China
Abstract:The complete mitochondrial genome (mitogenome) of Diaphania pyloalis (Lepidoptera: Pyralididae) was determined to be 15,298 bp and has the typical gene organization of mitogenomes from lepidopteran insects. It consists of 13 protein-coding genes (PCGs), two rRNA genes, 22 tRNA genes and an A + T-rich region. The A + T content of this mitogenome is 80.83% and the AT skew is slightly positive. All PCGs are initiated by ATN codons, except for cytochrome c oxidase subunit 1 (cox1) gene which is initiated by CGA. Only the cox2 gene has an incomplete stop codon consisting of just a T. All the tRNA genes display a typical clover-leaf structure of mitochondrial tRNA. The A + T-rich region of the mitogenome is 332 bp in length, including several common features found in lepidopteran mitogenomes. Phylogenetic analysis showed that the D. pyloalis is close to Pyralididae.
Keywords:PCR  polymerase chain reaction  ATPase  adenosine triphosphatase  NADH  nicotinamide adenine dinucleotide hydrogen
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