Further evidence for the contribution of the vascular endothelial growth factor gene in coronary artery disease susceptibility |
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Authors: | QT Cui Y Li CH Duan W Zhang XL Guo |
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Institution: | 1. Department of Cardiothoracic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan, PR China;2. Department of General Surgery, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan, PR China;3. Department of Operating room, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan, PR China |
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Abstract: | Coronary artery disease (CAD) receives intensive attentions in the research of cardiovascular diseases, due to its high incidence and severe impact on the quality of life vascular endothelial growth factor (VEGF), a potent angiogenic and vascular permeability factor, has been strongly implicated in the pathogenesis of CAD. Genetic markers in different regions of the VEGF gene have a plausible role in modulating the risk of CAD. To identify the markers contributing to the genetic susceptibility to CAD, we examined the potential association between CAD and 10 single nucleotide polymorphisms (SNPs, rs699947, rs1570360, rs2010963, rs833068, rs3024997, rs3025000, rs3025010, rs3025020, rs3025030, rs3025039) of the VEGF gene using the MassARRAY system. Participants included 242 CAD patients and 253 healthy controls from a Chinese Han Population (He'nan Province, China). The allelic or genotypic frequencies of the rs699947 (5′ untranslated regions, 5′UTR) and rs2010963 (5′UTR) polymorphisms in the CAD patients were significantly different from those in the healthy controls. The CAD patients had significantly higher frequency of the rs699947 A allele (χ2 = 11.141, P = 0.001, OR = 1.665, 95% CI = 1.232–2.250) and rs2010963 C allele (χ2 = 13.593, P = 0.0002, OR = 1.611, 95% CI = 1.249–2.077). Strong linkage disequilibrium was observed in the rs699947–rs1570360–rs2010963 haplotype block (D’ > 0.9). Significantly more C–G–C haplotypes (P = 0.040) and significantly fewer C–G–G haplotypes (P = 0.0004) were found in the CAD patients. The possible association of rs699947 and rs2010963 with CAD risks warrant confirmation in independent case–control studies and may be informative for future investigations on the pathogenesis of CAD. |
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Keywords: | AMI acute myocardial infarction CABG coronary artery bypass graft CAD coronary artery disease CHB Chinese Han in Beijing CI confidence interval EDTA ethylenediamine tetraacetic acid LPS lipopolysaccharide MAF minor allele frequency OR odds ratio PBMC peripheral blood mononuclear cell rhVEGF recombinant human vascular endothelial growth factor SAP stable angina pectoris SNP single nucleotide polymorphism UAP unstable angina pectoris UTR untranslated regions VEGF vascular endothelial growth factor |
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