Association of leptin receptor gene Q223R polymorphism on lipid profiles in comparison study between obese and non-obese subjects |
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Authors: | Eda Becer,Gü ldal Mehmetç ik,Halin Bareke,Nedime Serakıncı |
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Affiliation: | 1. Department of Biochemistry, Faculty of Pharmacy, Near East University, Nicosia, Mersin 10, Turkey;2. Department of Medical Biology and Genetics, Faculty of Medicine, Near East University, Nicosia, Mersin 10, Turkey |
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Abstract: | ObjectivesLeptin is a hormone secreted from adipocytes. It regulates metabolism and energy homeostasis through the leptin receptor (LEPR) which is localized centrally in hypothalamus as well as in peripheral tissues. The aim of this study was to investigate the association of leptin receptor gene Q223R polymorphism on obesity in association with body mass index (BMI), lipid parameters, plasma leptin levels and homeostasis model assessment of insulin resistance (HOMA-IR).Design and methodsThe study included 110 obese and 90 non-obese subjects. The LEPR Q223R polymorphism was determined by polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP). Plasma leptin levels, serum lipid and antropometric parameters were measured.ResultsNo association was found between LEPR gene Q223R polymorphism and BMI in both study and control groups. Strikingly study group with non-obese subjects and with the RR genotype (homozygous mutant) had significantly higher serum total cholesterol (p < 0.001) and low density lipoprotein cholesterol (LDL-cholesterol) levels (p < 0.05) than QR (heterozygous) and QQ (wild type) genotypes. In obese group, subjects with the RR genotypes had significantly higher triglycerides (p < 0.05) levels, waist (p < 0.05) and hip circumferences (p < 0.001) than the QQ and QR genotypes.ConclusionsOur results suggest that the LEPR gene Q223R polymorphism has an association with waist and hip circumferences in obese group but no direct association with obesity although there is a significant influence on lipid profile both in obese and non-obese subjects. |
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Keywords: | LEPR, leptin receptor BMI, body mass index HOMA-IR, homeostasis model assessment of insulin resistance PCR&ndash RFLP, polymerase chain reaction&ndash restriction fragment length polymorphism LDL-C, low density lipoprotein cholesterol SNPs, single nucleotide polymorphisms HMG-CoA reductase, 3-hydroxy-3-methylglutaryl coenzyme reductase VLDL, very-low density lipoprotein TG, triglycerides HDL-C, high-density lipoprotein cholesterol ELISA, enzyme-linked immunosorbent assay ANOVA, analysis of variance ApoB, apolipoprotein B |
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