Haplotypes and effects on growth traits of bovine Wnt7a gene in Chinese Qinchuan cattle |
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| Authors: | Jing Xue Yujia Sun Wenjiao Guo Ziqi Yang Huibin Tian Chunlei Zhang Chuzhao Lei Xianyong Lan Hong Chen |
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| Affiliation: | 1. College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China;2. Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou, Jiangsu 221116, China |
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| Abstract: | Wnt7a is a member of the WNT gene family, which encodes secreted signaling proteins and responds to many biological processes. Specifically Wnt7a influences satellite stem cells and regulates the regenerative potential of the muscle. However, similar researches about the bovine Wnt7a gene are lacking. Therefore, in this study, polymorphisms of the bovine Wnt7a gene were detected in 488 individuals from Chinese Qinchuan cattle by DNA pooling, forced PCR-RFLP, and DNA sequencing methods. 3 novel SNPs were identified, two SNPs (g.T4926C and g.A21943G) were in the intron and the last one (g.C63777T) was in the exon. Five haplotypes involved in these three variant sites in the Wnt7a gene were identified and their effects on growth traits were analyzed. The results revealed that haplotype 1 had the highest haplotype frequencies and was highly significantly associated with body height (P < 0.01), body weight (P < 0.05), chest width (P < 0.05) and height at hip cross (P < 0.01) respectively. |
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| Keywords: | Wnt7a, Wingless-type MMTV integration site family, member 7A PCR-RFLP, polymerase chain reaction-restriction fragment length polymorphism bp, base pairs SNP, single nucleotide polymorphisms HWE, Hardy&ndash Weinberg equilibrium PIC, polymorphism information content LD, Linkage disequilibrium EM, expectation maximization ANOVA, analysis of variance GLM, general linear model MAS, Marker-assisted selection |
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