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Lactoferricin enhances BMP7-stimulated anabolic pathways in intervertebral disc cells
Authors:Michael B. Ellman  Jaesung Kim  Howard S. An  Di Chen  Ranjan Kc  Xin Li  Guozhi Xiao  Dongyao Yan  Joon Suh  Andre J. van Wjnen  James H.-C. Wang  Su-Gwan Kim  Hee-Jeong Im
Affiliation:1. Department of Biochemistry, Section of Rheumatology, Rush University Medical Center, Chicago, IL 60612, USA;2. Department of Orthopedic Surgery, Section of Rheumatology, Rush University Medical Center, Chicago, IL 60612, USA;3. Department of Internal Medicine, Section of Rheumatology, Rush University Medical Center, Chicago, IL 60612, USA;4. Department of Bioengineering, University of Illinois at Chicago, IL 60612, USA;5. Center of Regenerative Medicine and Departments of Orthopedic Surgery & Biochemistry & Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA;6. MechanoBiology Laboratory, Departments of Orthopedic Surgery and Bioengineering, University of Pittsburgh, PA 15213, USA;g Department of Oral and Maxillofacial Surgery, School of Dentistry, Chosun University, GwangJu City 501-759, Republic of Korea
Abstract:
Keywords:Aa, amino acid   ADAMTS, a disintergrin and metalloproteinase with thrombospondin motifs   AF, annulus fibrosus   bFGF, basic fibroblast growth factor   BMP, bone morphogenetic protein   C-ABC, chondroitin-ABC   cDNA, DNA complementary to RNA   CM, cell-associated matrix   DDD, degenerative disc disease   DMEM, Dulbecco's Modified Eagle medium   DMMB, dimethylmethylene blue   DNA, deoxyribonucleic acid   ECM, extracellular matrix   GAG, glycosaminoglycan   IGF-1, insulin-like growth factor-1   IL-1, interleukin-1   iNOS, inducible nitric oxide synthase   IVD, intervertebral disc   LF, lactoferrin   LfcinB, lactoferricin B   MAPK, mitogen-activated protein kinase   MMP, matrix metalloproteinase   mRNA, messenger RNA   NP, nucleus pulposus   PG, proteoglycan   TGF-β, transforming growth factor-beta   TNF-α, tumor necrosis factor-alpha   RIPA, radio immunoprecipitation assay   RNA, ribonucleic acid   RT-PCR, real time polymerase chain reaction   siRNA, small interfering RNA   TIMP, tissue inhibitor of matrix metalloproteinase
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