Association between interleukin 4 gene intron 3 VNTR polymorphism and recurrent aphthous stomatitis in a cohort of Turkish patients |
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Authors: | Göknur Kalkan Serbulent Yigit Nevin Karakus Yalçın Baş Havva Yıldız Seçkin |
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Institution: | 1. Gaziosmanpasa University, Faculty of Medicine, Department of Dermatovenerology, Tokat, Turkey;2. Gaziosmanpasa University, Faculty of Medicine, Department of Medical Biology, Tokat, Turkey |
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Abstract: | ObjectiveRecurrent aphthous stomatitis (RAS) is one of the most common oral mucosal diseases, with a multifactorial etiopathogenesis, an interaction between predisposing factors and/or systemic conditions and immunological components in genetically predisposed subjects. Although there is no clear genetic mode of inheritance, there is evidence that inheritance of specific gene polymorphisms may predispose individuals to RAS. The purpose of the present study was to investigate a possible association between the functional interleukin 4 (IL4) VNTR genetic polymorphism and RAS in a sample of Turkish patients.MethodsThe study included 145 unrelated patients with a clinical diagnosis of RAS and 150 unrelated healthy controls. Genomic DNA was isolated and IL4 gene 70 bp VNTR polymorphism determined by using polymerase chain reaction (PCR) with specific primers.ResultsThe distribution of genotype and allele frequencies of IL4 gene intron 3 VNTR polymorphism was statistically different between RAS patients and control group (p < 0.0001 and p < 0.0001, respectively) P2P2 genotype and P2 allele were also found to be protective with a lower risk for susceptibility to RAS (p < 0.0001).ConclusionThe results of this study suggest that intron 3 VNTR polymorphism in the IL4 gene is associated with RAS susceptibility in Turkish population. |
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Keywords: | RAS Recurrent aphthous stomatitis IL interleukin PCR polymerase chain reaction VNTR variable number of tandem repeat RA rheumatoid arthritis ESRD end-stage renal disease SLE systemic lupus erythematosus MS multiple sclerosis SD standard deviation ANOVA analysis of variance OR odds ratio CI confidence interval GM-CSF granulocyte-macrophage colony-stimulating factor BD Behç et's disease |
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