Klotho gene polymorphism of rs3752472 is associated with the risk of urinary calculi in the population of Han nationality in Eastern China |
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Authors: | Chen Xu Ri-jin Song Jie Yang Bin Jiang Xiao-lan Wang Wei Wu Wei Zhang |
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Affiliation: | 1. State Key Laboratory of Reproductive Medicine, Department of Urology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, China;2. State Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing 210029, China;3. Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 210029, China |
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Abstract: | Background/aimsThe incidence of urolithiasis has considerably increased throughout the world in the last two decades. Clinical researches have showed an association between oxidative stress and stone formation. Emerging evidence indicated a novel function for klotho protein in anti-oxidative stress. In this study, we aimed at investigating a possible relationship between klotho gene polymorphisms and the risk of calcium oxalate urolithiasis in the population of Han nationality in Eastern China.MethodsKlotho gene polymorphisms rs3752472 in exon3, rs650439 in intron 4 and rs577912 in intron 1 were investigated in 426 patients with calcium oxalate stones compared with 282 age-matched healthy volunteers with no history of stone formation, using TaqMan SNP Genotyping Assays.ResultsSignificant differences were found between rs3752472 and the risk of nephrolithiasis as CC genotype of rs3752472 klotho polymorphism had almost 2-fold increased stone risk compared with the heterozygote genotype CT and homozygous genotype TT(95% CI = 1.013–2.255, OR = 1.512,p = 0.043).ConclusionOur results showed that the rs3752472 polymorphism of klotho gene is associated with the risk of calcium oxalate urolithiasis and may act as a risk factor during stone formation in our study population. |
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Keywords: | SNP, single nucleotide polymorphisms CaOx, calcium oxalate COM, calcium oxalate monohydrate COD, calcium oxalate dehydrate OS, oxidative stress ROS, reactive oxygen species Insulin/IGF-1, insulin/insulin-like growth factor-1 ESWL, extracorporeal shock wave lithotripsy PCNL, percutaneous nephrolithotomy Cr, creatinine LDH, lactic dehydrogenase DNA, deoxyribose nucleic acid EDTA, ethylene diamine tetraacetic acid PCR, polymerase chain reaction ORs, odds ratios CI, confidence interval pH, potential of hydrogen FOXO, forkhead box SOD2, manganese-superoxide dismutase ESRD, end-stage renal disease mRNA, messenger ribose nucleic acid |
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