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Significant differences in genotoxicity induced by retrovirus integration in human T cells and induced pluripotent stem cells |
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| Authors: | Weiyan Zheng Yingjia Wang Tammy Chang He Huang Jiing-Kuan Yee |
| Affiliation: | 1. Bone Marrow Transplantation Center, Department of Hematology, The First Affiliated Hospital, Zhejiang University, Hangzhou, China;2. Department of Virology, City of Hope National Medical Center, Duarte, CA, USA |
| Abstract: | |
| Keywords: | iPSCs, induced pluripotent stem cells ESC, embryonic stem cell LTR, long terminal repeat SMA, spinal muscular atrophy DMEM, Dulbecco's modified Eagle medium FBS, fetal bovine serum MEF, mouse embryonic fibroblasts EB, embryoid bodies FACS, fluorescence-activated cell sorting shRNA, small hairpin RNA LM-PCR, ligation-mediated PCR qRT-PCR, quantitative RT-PCR RTCGD, retrovirus tagged cancer gene database CIS, common integration site ssh2, slingshot 2 pmca2, plasma membrane calcium ATPase 2 UTR, untranslated region |
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