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Significant differences in genotoxicity induced by retrovirus integration in human T cells and induced pluripotent stem cells |
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| Authors: | Weiyan Zheng Yingjia Wang Tammy Chang He Huang Jiing-Kuan Yee |
| Institution: | 1. Bone Marrow Transplantation Center, Department of Hematology, The First Affiliated Hospital, Zhejiang University, Hangzhou, China;2. Department of Virology, City of Hope National Medical Center, Duarte, CA, USA |
| Abstract: | |
| Keywords: | iPSCs induced pluripotent stem cells ESC embryonic stem cell LTR long terminal repeat SMA spinal muscular atrophy DMEM Dulbecco's modified Eagle medium FBS fetal bovine serum MEF mouse embryonic fibroblasts EB embryoid bodies FACS fluorescence-activated cell sorting shRNA small hairpin RNA LM-PCR ligation-mediated PCR qRT-PCR quantitative RT-PCR RTCGD retrovirus tagged cancer gene database CIS common integration site ssh2 slingshot 2 pmca2 plasma membrane calcium ATPase 2 UTR untranslated region |
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