Molluskan fasciclin-1 domain-containing protein: Molecular characterizationand gene expression analysis of fasciclin 1-like protein from disk abalone (Haliotis discus discus) |
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Authors: | HKA Premachandra Mahanama De Zoysa Chamilani Nikapitiya Youngdeuk Lee WDN Wickramaarachchi Ilson Whang Jehee Lee |
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Institution: | 1. Department of Marine Life Sciences, School of Marine Biomedical Science, Jeju National University, Jeju Special Self-Governing Province 690-756, Republic of Korea;2. College of Veterinary Medicine, Chungnam National University, Yuseong-gu, Daejeon 305-764, Republic of Korea;3. Department of Aqualife Medicine, Chonnam National University, Chonnam 550-749, Republic of Korea;4. Marine and Environmental Institute, Jeju National University, Jeju Special Self-Governing Province 690-814, Republic of Korea |
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Abstract: | Cell-to-cell contacts play a key role in multicellular systems and organisms. Fasciclin-1 (FAS-1) is a lipid-linked membrane associated glycoprotein that is a member of a newly recognized family of cell adhesion molecules sharing features with the immunoglobulins, cadherins, integrins, and selectins. Here, we report the identification and molecular characterization of a novel FAS-1 domain-containing cDNA from disk abalone (Haliotis discus discus), including its gene expression profile and immune response to bacterial stimuli and tissue injuries. Designated as Abfac1, the 909 bp open reading frame (ORF) encodes 303 amino acid (aa) residues with a predicted molecular mass of 33 kDa and isoelectric (pI) value of 4.9. The aa sequence contains two FAS-1 domains and three conserved regions, FRa motif, H-box, and FRb motif. Phylogenetic analysis showed the closest relation to Jellyfish cell adhesion protein. In healthy abalone, Abfac1 expression is highest in hepatopancreas followed by mantle and lowest in digestive gland. In immune-stimulated abalones, relative Abfac1 mRNA expression was increased in hemocytes by ~ 11-fold at 48 h after the Vibrio parahaemolyticus infection, by 3.1-fold at 6 h after the Listeria monocytogenes infection and by ~ 9-fold at 6 h after the LPS injection. Similarly, tissue injuries caused significant increase of relative mRNA expression by 3.5-fold in hemocytes and by ~ 10-fold in mantle at 12 h post-injury. These results suggest that the novel member of the FAS-1 domain-containing protein family, Abfac1, may be involved in immune response and cell adhesion in disk abalone. |
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Keywords: | FAS-1 fasciclin-1 ORF open reading frame aa amino acid Abfac1 disk abalone fasciclin-1 domain containing gene/protein mRNA messenger ribonucleic acid mAb monoclonal antibody EST expressed sequence tag LPS lipopolysaccharide i m intramuscular injection p i post infection/induction cDNA complementary deoxyribonucleic acid qPCR quantitative real-time polymerase chain reaction bp base pair UTR un-translated region WSSV white spot syndrome virus ECM extracellular matrix HC hemocyte Gl gill Mn mantle Ms muscle Dg digestive gland Hp hepatopancreas |
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