Single nucleotide polymorphism of CD40 in the 5′-untranslated region is associated with ischemic stroke |
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Authors: | Ying Ma Shun-Xian Wang Yun Liu Guo-Guang Peng Xiao-Ming Wang Bo Zhang Bi-Hua Wu Ju-Ming Yu |
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Institution: | 1. Department of Neurology, Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, PR China;2. Department of Forensic Medicine, North Sichuan Medical College, Nanchong 637000, PR China;3. Department of Neurology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, PR China |
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Abstract: | ObjectivesIschemic stroke is influenced by both environmental and genetic factors. The CD40/CD40L system is related to proinflammatory and prothrombogenic responses, which are involved in the pathophysiology of ischemic stroke. The aim of this study was to evaluate association between the CD40 -1C/T single nucleotide polymorphism (SNP) and ischemic stroke in a Chinese population.MethodsWe conducted a case–control study including 286 ischemic stroke patients and 336 controls. CD40 -1C/T SNP was genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing methods, and evaluated its relevance to ischemic stroke susceptibility.ResultsSignificantly increased ischemic stroke risk was found to be associated with the T allele of CD40 -1C/T (OR = 1.273, 95% CI = 1.016–1.594). The frequencies of CT and TT/CT genotypes of CD40 -1C/T in ischemic stroke patients were significantly higher than those of controls, respectively (for CT: OR = 2.350, 95% CI = 1.601–3.449; for TT/CT: OR = 2.148, 95% CI = 1.479–3.119). And, similar results were obtained after adjusting non-matched variables. We found that the frequency of carried T genotypes (TT and TT/CT) was significantly increased in patients with history of stroke compared with patients without (for TT: OR = 6.538, 95%CI = 1.655–25.833; for TT/CT: OR = 3.469, 95%CI = 1.031–11.670), respectively.ConclusionsThe findings suggested that the CD40 -1C/T polymorphism might contribute to the susceptibility to ischemic stroke in the Chinese population, and might be associated with history of previous stroke. |
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Keywords: | SNP single nucleotide polymorphism PCR-RFLP polymerase chain reaction-restriction fragment length polymorphism TNFR tumor necrosis factor receptor CD40L CD40 ligand CNS central nervous system 5&prime -UTR 5&prime -untranslated region ICD-9-CM International Classification of Diseases 9th Revision Clinical Modification CT computed tomography MRI magnetic resonance imaging TOAST Trial of Org10172 in Acute Stroke Treatment LAA large artery atherosclerosis CE cardioembolism SVO small vessel occlusion NIHSS National Institutes of Health Stroke Scale OR odds ratio CI confidence intervals HWE Hardy&ndash Weinberg equilibrium CHD coronary heart disease LD linkage disequilibrium sCD40 soluble CD40 |
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