Genome-scale analysis of the metabolic networks of oleaginous Zygomycete fungi |
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Authors: | Wanwipa Vongsangnak Rawisara Ruenwai Xin Tang Xinjie Hu Hao Zhang Bairong Shen Yuanda Song Kobkul Laoteng |
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Affiliation: | 1. Center for Systems Biology, Soochow University, Suzhou 215006, China;2. School of Agriculture and Natural Resources, University of Phayao, Maeka, Muang, Phayao 56000, Thailand;3. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China;4. Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology, National Sciences and Technology Development Agency, Khong Luang, Pathum Thani 12120, Thailand |
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Abstract: | Microbial lipids are becoming an attractive option for the industrial production of foods and oleochemicals. To investigate the lipid physiology of the oleaginous microorganisms, at the system level, genome-scale metabolic networks of Mortierella alpina and Mucor circinelloides were constructed using bioinformatics and systems biology. As scaffolds for integrated data analysis focusing on lipid production, consensus metabolic routes governing fatty acid synthesis, and lipid storage and mobilisation were identified by comparative analysis of developed metabolic networks. Unique metabolic features were identified in individual fungi, particularly in NADPH metabolism and sterol biosynthesis, which might be related to differences in fungal lipid phenotypes. The frameworks detailing the metabolic relationship between M. alpina and M. circinelloides generated in this study is useful for further elucidation of the microbial oleaginicity, which might lead to the production improvement of microbial oils as alternative feedstocks for oleochemical industry. |
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Keywords: | ACP, acyl carrier protein ACOAA, acetyl-CoA acyltransferase ACC, acetyl-CoA carboxylase AOX, acyl-CoA oxidase ARA, arachidonic acid ACLY, ATP-citrate lyase CoA, Coenzyme A DGAT, diacylglycerol O-acyltransferase DGLA, dihomo γ-linolenic acid DHAPAT, dihydroxyacetone phosphate acyltransferase FADS3, ω3-fatty acid desaturase FADS5, Δ5-fatty acid desaturase FMN, flavin mononucleotide EPA, eicosapentaenoic acid FAS, fatty acid synthase G6PD, glucose-6-phospate 1-dehydrogenase GLA, γ-linolenic acid GLUD, glutamate dehydrogenase GPAT, glycerol-3-phosphate acyltransferase GPR, gene-protein-reaction ICDH, isocitrate dehydrogenase LPA, lysophosphatidic acid LPC, lysophosphatidylcholine LPAAT, lysophosphatidic acid acyltransferase MBOP, long-chain-enoyl-CoA hydratase ME, malic enzyme MCD, malonyl-CoA decarboxylase NADPH, nicotinamide-adenine dinucleotide phosphate PA, phosphatidic acid PC, phosphatidylcholine PE, phosphatidylethanolamine PG, phosphatidylglycerol PS, phosphatidylserine PI, phosphatidylinositol PDAT, phospholipid: diacylglycerol acyltransferase PDH, pyruvate dehydrogenase PPAP, phosphatidate phosphatase SE, sterol esterase SI, sterol isomerase SR, sterol reductase TAG, triacylglycerol TCA, tricarboxylic acid TGL, triglyceride lipase zHAD, 3-hydroxyacyl-CoA dehydrogenae |
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