Identification and genetic effect of haplotype in the bovine BMP7 gene |
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Authors: | Yong-Zhen Huang Xin-Lei Wang Hua He Xian-Yong Lan Chu-Zhao Lei Chun-Lei Zhang Hong Chen |
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Affiliation: | 1. College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China;2. Institute of Cellular and Molecular Biology, Jiangsu Normal University, Xuzhou, Jiangsu 221116, China |
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Abstract: | Bone morphogenetic proteins (BMPs) are peptide growth factors belonging to the transforming growth factor-beta (TGF-β) superfamily, and some members of the BMP family support white adipocyte differentiation. In this study, we focused on the BMP7 which singularly promotes the differentiation of brown preadipocytes. Haplotypes involving 5 single nucleotide polymorphism (SNP) sites in the bovine BMP7 gene were identified and their effect on body weight was analyzed. 16 haplotypes and 18 combined haplotypes were revealed and the linkage disequilibrium was assessed in the cattle population with 602 individuals representing three main cattle breeds from China. The results showed that haplotypes 3, 10 and 14 were predominant and accounted for 75.64%, 69.85%, and 83.36% in Nanyang, Qinchuan and Jiaxian cattle breeds, respectively. The statistical analyses indicated that the SNP 1, 4, and 5 are associated with the body weight, body length, and heart girth at 12 and 24 months in Nanyang cattle population (P < 0.05), whereas there is no significant association between their 16 haplotypes and 18 combined haplotypes. Our results provide evidence that some SNPs and haplotypes in BMP7 are associated with growth traits, and may be utilized as a genetic marker in marker-assisted selection for beef cattle breeding programs. |
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Keywords: | bp, base pair(s) BMPs, Bone morphogenetic proteins CH, Chinese Holstein GLM, general linear models He, heterozygosity HWE, Hardy&ndash Weinberg equilibrium JX, Jiaxian LD, linkage disequilibrium LSM, least square means Ne, effective allele numbers NY, Nanyang PAGE, polyacrylamide gel electrophoresis PCR&ndash SSCP, polymerase chain reaction&ndash single stranded conformational polymorphism PIC, polymorphism information content QC, Qinchuan SNPs, single nucleotide polymorphisms SPSS, statistical product and service solutions TBE, is a buffer solution containing a mixture of Tris base, boric acid and EDTA TGF-β, transforming growth factor-beta UCP1, uncoupling protein 1 |
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