Iridium oxide pH microelectrode |
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Authors: | Vanhoudt P Lewandowski Z Little B |
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Institution: | National Science Foundation Research Center for interfacial Microbial Process Engineering, Montana State University, Bozeman, Montana 59717. |
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Abstract: | The manufacture, calibration, and signal conditioning during construction of an iridium/iridium oxide pH microsensor is described. The microsensor was designed to be used extracellularly, primarily in biofilm research. The sensing tip diameters were typically in the range of 3-15 mum. The iridium oxide was formed by potential cycling in dilute sulfuric acid. A pH profule across a denitrifying biofilm was measured as an example of an application. The higher Nernstian slope (70-80 mV/pH for fresh electrodes), increased rigidity, and restriction of the sensing tip to the outermost end of the electrode are features which make the iridium/iridium oxide pH microelectrode superior to a glass microelectrode. (c) 1992 John Wiley & Sons, Inc. |
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Keywords: | pH measurement microelectrodes microenvironments biofilms |
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