The prestalk/prespore differentiation and polarized cell movement inDictyostelium discoideum slugs. A possible involvement of the intracellular Ca2+-concentration |
| |
Authors: | Tomoaki Abe Yasuo Maeda |
| |
Affiliation: | (1) Biological Institute, Faculty of Science, Tohoku University, Aoba, Sendai |
| |
Abstract: | Summary Intracellular free calcium ion concentrations ([Ca2+]i) in the anterior prestalk and posterior prespore cells of theDictyostelium discoideum slug were determined, using the highly selective Ca2+ indicators, quin-2/AM and fura-2/AM. Temporal changes in [Ca2+]i in response to chemotactic stimulation with cAMP were also monitored at the single-cell level and compared between the two types of cells. The results obtained showed that resting [Ca2+]i in the prestalk cells is considerably higher than that in the prespore cells. Moreover, transient increase in [Ca2+]i upon stimulation with a low concentration of cAMP (20 nM) was noticed only in the prestalk cells, but not in the prespore cells. These facts are discussed in relation to the polarized movement and cellular differentiation in the migrating slug.Abbreviations cAMP 3,5-cyclic adenosine monophosphate - DIF differentiation-inducing factors - IP3 inositol 1,4,5-triphosphate |
| |
Keywords: | Dictyostelium discoideum Cellular slime moulds Calcium Cyclic AMP Chemotaxis Differentiation Slug movement |
本文献已被 SpringerLink 等数据库收录! |
|