Germplasm conservation of Guazuma crinita,a useful tree in the Peru-Amazon,by the cryopreservation of in vitro-cultured multiple bud clusters |
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Authors: | Maruyama Emilio Kinoshita Isao Ishii Katsuaki Ohba Kihachiro Sakai Akira |
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Affiliation: | (1) Institute of Agriculture and Forestry, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki 305, Japan;(2) Bio-Resources Technology Division, Forestry and Forest Products Research Institute, P.O. Box 16, Tsukuba Norinkenkyu Danchi-Nai, Ibaraki 305, Japan;(3) Asabucho 1-5-23, Kitaku, Sapporo 001, Japan |
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Abstract: | In vitro-cultured adventitious bud clusters of Guazuma crinita Mart. were successfully cryopreserved by the one-step vitrification method. Small segments (1.0-1.5 mm3) cut from adventitious bud clusters were exposed to a cryoprotectant mix solution containing (w/v), 25 glycerol, 15 sucrose, 15 ethylene glycol, 13 dimethyl sulfoxide, and 2 polyethylene glycol, at 25 °C for 15-60 min prior to storage in liquid nitrogen. After rapid warming (37 °C), the segments were treated with woody plant medium containing 40 (w/v) sucrose for 20 min at 25 °C, and then transferred to recovery-growth medium. High survival rates (about 80) were achieved without any cold hardening and/or pre-culturing treatments, and about 30 of the surviving cryopreserved explants regenerated plants. This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | adventitious buds bolaina blanca long-term storage Sterculiaceae tropical tree vitrification |
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