Cerato-platanin, the first member of a new fungal protein family |
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Authors: | Luigia Pazzagli Barbara Pantera Lara Carresi Camilla Zoppi Thelma A Pertinhez Alberto Spisni Stefania Tegli Aniello Scala Gianni Cappugi |
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Institution: | (1) Dipartimenti di Scienze Biochimiche, Università di Firenze, Firenze, Italy;(2) Dipartimenti di Biotecnologie Agrarie, Università di Firenze, Firenze, Italy;(3) Laboratorio Nacional de Luz Sincroton, Centro de Biologia Molecular Estructural, Campinas, Brazil;(4) Dipartimento di Medicina Sperimentale, Università di Parma, Parma, Italy |
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Abstract: | The ascomcete Ceratocystis fimbriata, the causal agent of “canker stain disease,” secretes a protein of 12.4 kDa that elicits phytoalexin synthesis and plant
cell death. This protein, named cerato-platanin (CP), is also located in the cell walls of ascospores, hyphae, and conidia;
it contains four cysteines (S-S bridged) and is moderately hydrophobic. The cp gene consists of a single exon and has 42 bp codifying for a signal peptide of 14 residues. The recombinant protein was obtained
by cloning the cp gene of the mature protein in Escherichia coli (BL21), and a refolding step was needed to achieve the native active form. In the European Molecular Biology data bank, CP
is reported as the first member of the CP family; this is the first example of an set of secreted fungal proteins whose primary
structure is very similar. Nonetheless, the data also revealed some structural and functional features that make CP simlar
to proteins of the hydrophobin family. |
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Keywords: | Cerato-platanin family hydrophobin cloning protein expression eliciting activity refolding |
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