长链非编码RNAIFNG-AS1靶向miR-19b-1-5p调控oxLDL诱导的血管内皮细胞增殖、凋亡的机制研究

为了探讨长链非编码RNA干扰素活化基因的反义核糖核酸(lncRNA IFNG-AS1)对氧化型低密度脂蛋白(oxLDL)诱导的人脐静脉血管内皮细胞EVC-304增殖、凋亡的影响和调控机制,该研究采用100 μg/mL的oxLDL分别处理转染si-IFNG-AS1、miR-19b-1-5p mimics或共转染si-IF...

Long Non-Coding RNA IFNG-AS1 Regulates oxLDL-Induced Proliferation and Apoptosis of Vascular Endothelial Cells by Targeting miR-19b-1-5p

To investigate the effect and mechanism of lncRNA IFNG-AS1 on the proliferation and apoptosis of human umbilical vein endothelial cells EVC-304 induced by oxLDL,100μg/mL oxLDL was used to treat the EVC-304 cells transfected with si-IFNG-AS1,miR-19b-1-5p mimics or co-transfected with si-IFNG-AS1 and anti-miR-19b-1-5p.Then,qRT-PCR was used to detect the expression of IFNG-AS1 and miR-19b-1-5p in the cells.CCK-8 method was used to detect cell proliferation,and flow cytometry was used to detect cell apoptosis.Western blot was used to detect the protein expression of P21,P16,cleaved-PARP and cleaved-caspase-3.Dual luciferase reporter gene experiment was applied for verifying the targeting relationship between IFNG-AS1 and miR-19b-1-5p.The results showed that oxLDL promoted the expression of IFNG-AS1 in EVC-304 cells,but inhibited the expression of miR-19b-1-5p(P<0.05);IFNG-AS1 inhibition or miR-19b-1-5p overexpression increased the proliferation activity of EVC-304 cells induced by oxLDL and the protein expression of P21 and P16,while reduced the apoptosis rate and the protein expression of cleaved-PARP and cleaved-caspase-3(P<0.05);miR-19b-1-5p inhibition reversed the effect of IFNG-AS1 inhibition on the proliferation and apoptosis of EVC-304 cells induced by oxLDL;dual luciferase reporter gene experiments confirmed that IFNG-AS1 negatively regulated the expression of miR-19b-1-5p.These results suggested that inhibition of IFNG-AS1 might enhance the proliferation of EVC-304 cells treated with oxLDL and inhibit their apoptosis,and its action mechanism was related to the up-regulation of miR-19b-1-5p.The IFNG-AS1/miR-19b-1-5p axis may provide new targets for the treatment of atherosclerosis.